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KMID : 0923620110110020100
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Immune Network
2011 Volume.11 No. 2 p.100 ~ p.106
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Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen
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Jeon Ji-Yeong
Jung Kyung-Min
Chang Jun
Choi Eun-Young
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Abstract
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Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect.
Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/H-2Kb and analyzed for TCR sequences.
Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/H-2Kb with t1/2 values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were V?12D-3-01＋J?11-01 and V?12-1-01＋D?1-01 ＋J2-7-01 for clone #14, V?13D-1-02＋J?34-02 and V?13-1-02＋D?2-01＋J?2-7-01 for clone #15, and V?16D＋J?45-01 and V?12-1-01＋D?1-01＋J?2-5-01 for clone #23.
Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.
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KEYWORD
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CTL clone, Proliferation, Cytotoxicity, Avidity, TCR usage
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